Metoprolol Succinate (MS) is B1 -Blocker acting as a adrenoreceptor antagonist agent and Clopidogrel bisulphate (CLOP) is an antiplatelet agent so, the combination is used in the treatment of Hypertension who need antiplatelet therapy. MS and CLOP were estimated at 254 nm by densitometry using silica gel 60/UV254 as stationary phase and a premix of Hexane: Toluene: Methanol: TEA (5: 3: 1.2: 0.5 /v/v/v) as mobile phase. Rf value of MS and CLOP were 0.23±0.02 and 0.68±0.02 respectively, with a Linearity range 1000-3500 ng/band and 1500-5250 ng/band and R2 value was found to 0.999 for MS and CLOP both. The developed high performance thin layer chromatography method was found to be specific, precise and reproducible and can be used for the routine estimation of MS and CLOP in the combined tablet dosage form.
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